Media and OD reading


This week I made 8 250ml TGY media. 7 different bacteria were inoculated at 28 degrees celsius. They were then placed in the incubator shaker for 24 hours. 


Cell culture growth was measured at 600nm absorbance. I used the spectrometer to make sure the bacteria had a 0.1 absorbance. This step was taken to ensure that the bacteria was in their exponential growth phase.





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